Research Technician II

SHIFT:
Day (United States of America)

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A Brief Overview
This entry-level research technical role participates in a planned research projects by performing research protocols, experimental procedures and other laboratory tasks under direct supervision. May occasionally exercise minimal discretion and judgment in execution and interpretation of experiments, and of data derived.

The Sengupta Laboratory at the Children's Hospital of Philadelphia (CHOP) is seeking an enthusiastic and motivated researcher to join our team investigating how circadian rhythms regulate lung inflammation, injury, and repair.

Our laboratory studies how biological clocks influence immune responses to respiratory infections, tissue regeneration, and long-term pulmonary health. We combine molecular and cellular biology, immunology, genetics, and experimental models of lung injury to address fundamental questions with important implications for human disease.

This is an exciting opportunity to contribute to an active and collaborative research program at the intersection of circadian biology, immunology, and pulmonary medicine.

What you will do

- Performing molecular and cellular biology techniques, including flow cytometry, RNA isolation, qPCR, protein isolation, and Western blotting.
- Maintaining genetically modified mouse colonies, including breeding, genotyping, and associated regulatory documentation.
- Participating in animal experiments investigating respiratory infections, inflammation, and lung repair, with microscopy and image analyses.
- Learning and applying specialized techniques such as cell sorting, organoid assays, viral titration, and advanced immunophenotyping.
- Contributing to experimental design, data analysis, and scientific discussions.
- Train and supervise undergraduate students in the lab.
- Participate in joint lab meetings and seminar series with immunology, pulmonology, and circadian biology groups on campus.
- Performs routine tests / experiments under the direction of principal investigator, supervisor or other designee
- Performs research procedures on experimental samples as directed
- Records and checks experiments/ results for completeness and accuracy
- Performs calculations and maintains appropriate log and lab notebooks
- Performs tasks that require familiarity with fundamental techniques and lab equipment
- May assist with planning specific research protocols and procedures
- Maintains office, lab area, solutions, reagents, supplies and equipment, disposes of specimens, media and other materials (using appropriate procedures)
- Assists in care and maintenance of lab equipment
- May coordinate schedules of tests and procedures
- Attends courses, seminars, and lab meetings to expand knowledge of research and laboratory techniques
- Records results, organizes data and maintains lab notebook in an organized manner
- Compiles and helps analyze data, prepares charts, graphs, and results of studies as directed
- Conducts literary searches as directed

Education Qualifications

- High School Diploma / GED - Required
- Associate's Degree - Preferred
- Bachelor's Degree - Preferred

Experience Qualifications

- At least one (1) year of related classroom experience or successful completion of a technical training program (eg. Wistar, WPSI) and research laboratory experience - Required
- At least two (2) years of related classroom experience or successful completion of a technical training program (eg. Wistar, WPSI) and research laboratory experience - Preferred

Skills and Abilities

- Intermediate understanding of lab procedures, protocols, and equipment.
- Basic exposure to lab specific experiments / techniques and scientific analysis
- Excellent verbal and written communications skills
- Excellent organizational skills
- Solid critical thinking / problem-solving skills
- Solid analytical skills

Opportunities for Scientific Growth

We believe that research staff are integral members of the scientific team. Depending on interests, experience, and demonstrated proficiency, individuals will have opportunities to take ownership of research projects, participate in experimental planning and interpretation, present findings at scientific meetings, and contribute to peer-reviewed publications.

The CHOP and University of Pennsylvania research environment also offers access to a vibrant scientific community, including seminars, workshops, and interactions with investigators across multiple disciplines.

For early-career applicants, we encourage a commitment of approximately two years to allow meaningful engagement with research projects and professional development.

Selected Publications

Our laboratory's research spans fundamental circadian mechanisms, pulmonary inflammation, and tissue repair. Selected publications include:

- Sengupta S et al.  Circadian regulation of lung inflammation in influenza infection.  Nature Communications . 2019;10:4107.
- Issah Y et al.  Loss of Circadian Protection in Adults Exposed to Hyperoxia as Neonates.  eLife . 2021;10.
- Naik A et al.  Circadian regulation of lung repair and regeneration.  JCI Insight . 2023;8.
- Paul et al, JCI 2025

Additional recent publications and preprints from 2025–2026 to be inserted following bibliographic verification

Learn More

Laboratory website:   https://www.research.chop.edu/sengupta-laboratory

Bluesky:  @shaonsengupta.bsky.social

To carry out its mission, CHOP is committed to supporting the health of our patients, families, workforce, and global community. As a condition of employment, CHOP employees who work in patient care buildings or who have patient facing responsibilities must receive an annual influenza vaccine. Learn more.

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SALARY RANGE:

$43,900.00 - $54,800.00 Annually

Salary ranges are shown for full-time jobs. If you're working part-time, your pay will be adjusted accordingly.

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At CHOP, we are committed to fair and transparent pay practices. Factors such as skills and experience could result in an offer above the salary range noted in this job posting. Click here for more information regarding CHOP's Compensation and Benefits.