About this role
When you join the growing BILH team, you're not just taking a job, you’re making a difference in people’s lives.
The Kulkarni laboratory studies the biology and function of pancreatic islets including insulin secreting beta cells and glucagon-secreting alpha cells. The lab has recently started to work on adult tissue-specific stem cells, particularly those stem cells that form the pancreatic islet cells.
Job Description:
- Participate in flow cytometry and stem cell research involving the operation of high-speed, multi-parameter cell sorters and analyzers (complex, expensive optical/electronic instruments).
- Assist other lab members (Fellows) with culturing human tissues (e.g. skin and blood cells) including culturing, maintenance, and banking of cell lines.
- Assist lab members with mouse tissue cultures, maintenance and banking of cell lines.
- Routine culture of pluripotent stem cells, including picking of colonies, expansion, freezing of cells and generation of mouse embryonic feeder cells.
- Characterization of pluripotent stem cells, including teratoma assays, immunohistochemistry, qPCR and western blotting analyses.
- Screen offspring of several mouse strains by preparation of genomic DNA and PCR or by isolation of blood cells and flow cytometry.
- Assist in placement of orders for reagents and supplies as needed, log delivery of supplies and store supplies appropriately upon delivery.
- Assist with the maintenance of lab plasmid, antibody, and animal databases.
- Assist in the isolation of cells and tissues from experimental animals, including preparation of these cells for subsequent analysis by flow cytometry or immunohistochemistry.
- Assist in the isolation of cells from human tissue samples, including preparation of these cells for subsequent analysis by flow cytometry or immunohistochemistry.
- Assist with experimental procedures involving mice, including cell transplantation and injection of soluble factors, as well as routine monitoring for health.
- Work directly with a number of researchers, assisting in design, execution, and interpretation of experiments and also will assist in training and advising other lab members in use of equipment.
- Operating and maintaining equipment, scheduling procedures, working with multiple investigators, interfacing with vendors, and preparing reagents.
- Maintaining different animal strains, genotyping, injection, and other physiological experiments.
- Other duties as assigned.
Pay Range:
$15.00 - $24.00
The pay range listed for this position is the base hourly wage range the organization reasonably and in good faith expects to pay for this position at this time. Actual compensation is determined based on several factors, that may include seniority, education, training, relevant experience, relevant certifications, geography of work location, job responsibilities, or other applicable factors permissible by law. Compensation may exceed the base hourly rate depending on shift differentials, call pay, premium pay, overtime pay, and other additional pay practices, as applicable to the position and in accordance with the law.